Lignocellulosic biomass is an interesting option as a source of fermentable sugars for the production of biofuels and biochemicals considering its abundant availability and low-cost compared to commercially available refined sugars. One such major lignocellulosic biomass available in Malaysia is empty fruit bunches (EFB) which is a by-product from the palm oil milling process. Besides, the oil palm plantations also generate a large amount of oil palm trunk (OPT) and oil palm frond (OPF) during replanting and pruning. The annual production of EFB, OPT and OPF is approximately 84.23 million tonne (dry basis) a year (Table 1) from 5.74 million hectare of oil palm planted area (MPOB, 2016). This huge amount (i.e. 7 million t of EFB, 21.4 million t of OPT and 55.8 million t of OPF) implies that oil palm biomass is a readily available feedstock to provide intermediate platforms (xylose, C5 and glucose, C6 sugars) for the production of biofuels and biochemicals.